Mecanismos transcripcionales y epigenéticos de la plasticidad neuronal

2009

• Sanchis-Segura C*, Lopez-Atalaya JP* and Barco A (2009). Selective boosting of transcriptional and behavioral responses to drugs of abuse by histone deacetylase inhibition. Neuropsychopharmacology 34(13): 2642-54. * Equal contributors. (Featured article )
• Sanchis-Segura C, Jancic D, Jimenez-Minchan M and Barco A (2009). Inhibition of cAMP responsive element binding protein in striatal neurons enhances approach and avoidance responses towards morphine- and morphine withdrawal-related cues. Front Behav Neurosci 3:30 Open access frontiers
• Viosca J *, Lopez de Armentia M *, Jancic D and Barco A (2009). Enhanced CREB-dependent gene expression increases the excitability of neurons in the basal amygdala and primes the consolidation of contextual and cued fear memory. Learn Mem 16(3): 193-7.* Equal contributors
• Viosca J, Malleret G, Bourtchouladze R, Benito E, Vronskava S, Kandel ER and Barco A (2009). Chronic enhancement of CREB activity in the hippocampus interferes with the retrieval of spatial information. Learn Mem 16(3): 198-209 (issue cover).
• Jancic D*, Lopez de Armentia M*, Valor LM, Olivares R and Barco A (2009). Inhibition of cAMP-response element binding protein reduces neuronal excitability and plasticity, and triggers neurodegeneration. Cereb Cortex 19(11): 2535-47. * Equal contributors.
• Barco A (2009). Circuitos moleculares implicados en la formación de recuerdos. Chapter in La demencia: epidemia que crece (Ed. Fundación Neurociencias y Envejecimiento).
• Viosca J, Schuhmacher A, Guerra and Barco A (2009). Germ line expression of H-RasG12V causes neurological deficits associated to Costello syndrome. Genes Brain Behav 8(1): 60-71.

2008

• Barco A, Lopez de Armentia M and Alarcon JM (2008). Synapse-specific stabilization of plasticity processes: The synaptic tagging hypothesis revisited ten years later. Neurosci Biobehav Rev 32(4):831-51.
• Hawkins RD, Bailey H, Barco A and Kandel ER (2008). Molecular studies of learning and memory in Aplysia and the hippocampus: A comparative analysis of implicit and explicit memory storage. Chapter in “Learning and memory: A comprehensible reference” (Elsevier Books).

2007

• Lopez de Armentia M,Jancic D, Olivares R, Alarcon ER, Kandel ER and Barco A (2007).CREB-mediated gene expression increases the intrinsic excitability of CA1 pyramidal neurons. J Neurosci 27(50): 13909-13918.
• Barco A, Jancic D and Kandel ER (2007). CREB-dependent transcription and synaptic plasticity. Chapter in “Regulation of transcription by neuronal activity: To the nucleus and back” (Springer Science publishers).
• Barco A and Lopez de Armentia M (2007). El cerebro plástico. Chapter in Unidad didáctica “Viaje al Universo Neuronal”(FECYT).
• Barco A (2007). The Rubinstein-Taybi syndrome: Modeling mental impairment in the mouse. Genes, Brain & Behavior 6(S1):32-39.

2006

• Gasic GP, Barco A, Avila J and Lerma J (2006). A meeting to remember: Meeting on memory and related disorders. EMBO Reports 7(8):768-73.
• Barco A, Bailey CH and Kandel ER (2006). Common molecular mechanisms in explicit and implicit memory. J Neurochem 97(6):1520-33.
• Alarcon JM, Barco A and Kandel ER (2006). Capture of L-LTP within and across the apical and basilar dendritic compartments of CA1 pyramidal neurons: synaptic tagging is restricted to specific dendritic compartments. J Neurosci 26(1):256-264

2005

• Barco A and Kandel ER (2005). Role of CREB and CBP in brain function. Chapter in Transcription factors in the nervous system: Development, brain function and disease (Wiley-VCH publishers).
• Levine AA, Guan Z, Barco A, Xu S, Kandel ER and Schwartz JH (2005). CREB binding protein controls response to cocaine by acetylating histones at the fosB promoter in the mouse striatum. Proc Natl Acad Sci U S A. 102(52):19186-91.
• Barco A, Patterson S, Alarcon JM, Gromova P, Mata-Roig M, Morozov A and Kandel ER (2005). Gene expression profiling of facilitated L-LTP in VP16-CREB mice reveals that BDNF is critical for both the maintenance of LTP and for synaptic capture. Neuron, 48(1): 123-137.

2004

• Calandria C, Irurzun A, Barco A and Carrasco L (2004).Individual expression of poliovirus 2APRO induces caspase-3 activation and PARP cleavage in HeLa cells. Virus Research 104(1): 39-49.
• Alarcon JM, Malleret G, Touzani K, Vronskaya S, Ishii S, Kandel ER and Barco A (2004). Chromatin acetylation, memory, and LTP are impaired in CBP+/- mice: a model for the cognitive deficit in Rubinstein-Taybi syndrome and its amelioration. Neuron 42(6):947-959 (issue cover) (Featured article )
• Pham TA, Graham SJ, Suzuki S, Barco A, Kandel ER, Gordon B and Lickey ME (2004). A semi-persistent adult ocular dominance plasticity in visual cortex is stabilized by activated CREB. Learning and memory 11(6): 738-747.
• Gao Y, Deng K, Hou J, Bryson JB, Barco A, Nikulina E, Spencer T, Mellado W, Kandel ER and Filbin MT (2004). Activated CREB is sufficient to overcome inhibitors in myelin and promote spinal axon regeneration in vivo. Neuron 44(4):609-621.
• Lang C, Barco A, Zablow L, Kandel R, Siegelbaum SA, Zakharenko S (2004). Transient expansion of synaptically connected dendritic spines upon induction of hippocampal long-term potentiation. Proc Natl Acad Sci U S A. 101(47):16665-70.

2003

• Barco A, Pittenger C and Kandel ER (2003).CREB, memory enhancement and the treatment of memory disorders: promises, pitfalls and prospects. Expert Opinion on Therapeutic Targets 7(1):101-14.

2002

• Barco A, Alarcon JM and Kandel ER (2002) Expression of constitutively active CREB protein facilitates the late phase of long-term potentiation by enhancing synaptic capture. Cell 108(5):689-703.
• Agirre A, Barco A, Carrasco L and Nieva JL (2002) Viroporin-mediated membrane permeabilization: pore formation by non-structural poliovirus 2B protein. J Biol Chem 277(43):40434-40441.
• Carrasco L, Guinea R, Irurzun A and Barco A. (2002) Effects of viral replication on cellular membrane metabolism and function. Chapter in «MolecularBiology of Picornavirus» edited by B.L. Semler and E. Wimmer (ASM Press)

2000

• Barco A, Feduchi E and Carrasco L. (2000) Poliovirus Protease 3C(pro) Kills Cells by Apoptosis. Virology 266:352-360.
• Barco A, Feduchi E and Carrasco L. (2000) A stable HeLa cell line that inducibly expresses poliovirus 2Apro: effects on cellular and viral gene expression. J Virol 74: 2383-2392.

1999

• Ventoso I, Barco A and Carrasco L. (1999) Genetic selection of poliovirus 2Apro-binding peptides. J Virol 73:814-818.

1998

• Barco A. and Carrasco L. (1998) Identification of regions involved in cytotoxicity in poliovirus 2BC protein. J. Virol. 72: 3560-3570.
• Barco A. and Carrasco L. (1998) Co-expression of human eIF-4G and poliovirus 2Apro in Saccharomyces cereviseae Effects on gene expression. J Gen Virol 79:2651-60.
• Ventoso I, Barco A and Carrasco L. (1998) Mutational analysis of poliovirus 2Apro distinct inhibitory functions of 2Apro on translation and transcription. J Biol Chem 273:27960-27967.

1997

• Barco A, Ventoso I and Carrasco L. (1997). The yeast Saccharomyces cerevisiae as a genetic system for obtaining variants of poliovirus protease 2A. J Biol Chem 272: 12683-12691.

1996

• Aldabe R, Barco A and Carrasco L. (1996).Membrane permeabilization by poliovirus protein 2B and 2BC. J Biol Chem 271: 23134-23137.

1995

• Barco A and Carrasco L. (1995). Cloning and inducible synthesis of poliovirus non-structural proteins in Saccharomyces cerevisiae. Gene 156: 19-25.
• Barco A and Carrasco L. (1995). A human virus protein, poliovirus protein 2BC, induces membrane proliferation and blocks the exocytic pathway in the yeast Saccharomyces cereviseae. The EMBO J 14: 3349-3364.
• Barco A and Carrasco L. (1995). Poliovirus 2Apro expression inhibits growth of yeast cells. FEBS Letters 371: 4-8.

Financiación actual:

Nuestra investigación se financia gracias al apoyo de distintas organizaciones, públicas y privadas, nacionales e internacionales. En la actualidad recibimos fondos del MINECO, la Generalitat Valenciana, la Fundación Alicia Koplowitz, y la fundación americana Brain & Behavior Research Foundation.

Financiación previa por parte de las siguientes instituciones y programas:

Alumni, visitors and former members:

• Samanta Ortuño graduada en Biotecnología en la Universidad Miguel Hernández de Elche (UMH), con los Másteres en Biotecnología y Bioingeniería (UMH) y en Bioinformática (Universidad de Murcia). Realizó el trabajo de fin de Grado y Máster en biología molecular en el laboratorio del Dr. Antonio Vera (UMH). Continuó su experiencia trabajando en Metabarcoding y Bioinformática en la empresa Taxon Estudios Ambientales S.L. y en el laboratorio del Dr. José Antonio García Charton (Universidad de Murcia).
• Ana Martín estudió Biología en la Universidad de Málaga. Gracias al apoyo de una beca internacional de doctorado Marie Curie fellowship, Ana está desarrollando su proyecto de doctorado en el marco de una colaboracion entre el grupo del Prof. Wilczynski en el Nencki Institute of Experimental Biology (Varsovia) y nuestro laboratorio.
• Mayte López Cascales es licenciada en Bioquímica por la Universidad de Murcia, con un Máster de Neurociencias por la Universidad Pablo de Olavide de Sevilla, y Máster de Bioinformática en la Universidad de Murcia. En 2014 recibió el premio al mejor trabajo fin de master de bioinformatica por la universidad de Murcia. Actualmente está realizando su tesis doctoral en el grupo de la Dra. Herrera y trabaja en varios proyectos colaborativos entre los dos laboratorios.
• Jordi Fernández studied Biology at the Universitat de Girona (UdG) and got his Interuniversity Master degree in Neuroscience coordinated by the Universitat de Barcelona (UB). He was first trained in neurosciences and in molecular and cell biology techniques in the laboratories of Dr. Gemma Huguet (School of Science, UdG), Dr. Carles Sindreu (School of Medicine, UB) in which he conducted his master thesis, and Dr. Manuel Irimia (Centre de Regulació Genòmica, CRG). He joined our group in February 2015 thanks to a fellowship awarded by the Severo Ochoa program (MINECO). He defended his Ph.D. thesis in October 2019 and obtained the maximal qualification. Thesis: Immediate and deferred epigenomic signatures of in vivo neuronal activation in mouse hippocampus. Publications with us: Fernandez-Albert et al., Nat Neurosci 22, 1718-30; Lipinski et al., Nat Comm 11(1):2588. Other positions: Lab. Dr. Mark Schnitzer. University of Stanford, San Francisco, San Francisco, USA.
• Michal Lipinski estudió Biotecnología en la Universidad de Varsovia de Ciencias de la Vida (Polonia) y ha realizado estancias en laboratorios del Instituto Nencki de Biología Experimental (Varsovia) y del Departamento de Anatomía de la Universidad de Zurich. En Junio del 2012 se incorporó a nuestro grupo para realizar su tesis doctoral gracias a una beca financiada por el programa de Becas Santiago Grisolia de la Generalitat Valenciana. He defended his Ph.D. thesis in December 2019 and obtained the maximal qualification. Thesis: Role of CBP and p300 in the establishment and maintenance of transcriptional programs in adult excitatory neurons. Publications with us: Ito et al., Nat Comm 5:4450; Galvao-Ferreira et al., eNeuro 4(2); Scandaglia et al. Cell Reports 21(1):47-59; del Blanco et al., Cell Death Differ 26(11):2208-2222; Lipinski et al., Curr Opin Neurobiol 59:1-8;Fernandez-Albert et al., Nat Neurosci 22, 1718-30; Lipinski et al., Nat Comm 11(1):2588. Other positions: Lab. Dr. Paola Arlotta & Aviv Regev. Harvard Department of Stem Cell and Regenerative Biology, Cambridge & BROAD Institute. MA, USA.
• Marilyn Scandaglia studied Molecular Biotechnology at the Università Alma Mater Studiorum di Bologna in Italy. In 2011, as a pregraduate student, she moved to the Instituto de Neurociencias de Alicante and joined our lab to carry out her Master and PhD theses. She defended her Ph.D. thesis in January 2018 and obtained the maximal qualification. She also received the special distinction (Premio Extraordinario). Thesis: Role of lysine demethylase 5C in neurodevelopment and intellectual disability. Publications with us: Fiorenza et al., Cereb Cortex 26(4):1619-33; Scandaglia et al., Sci Reports5:17470; Iwase et al., J Neurosci 37(45):10773-10782; Scandaglia et al. Cell Reports 21(1):47-59; del Blanco et al., Cell Death Differ 26(11):2208-2222; Scandaglia and Barco. J Med Gen 56(8):491-498. Other positions: Lab. Dr. Berta Lopez Sanchez-Laorden. Instituto de Neurociencias (UMH-CSIC), Alicante, Spain.
• Anna Fiorenza studied Biology at the Università degli studi del Sannio in Italy. In 2008, she moved to the Instituto de Neurociencias de Alicante, as part of the «Erasmus Placement» subprogram in the U.E. Lifelong Learning Programme, where she worked in the lab of Dr. Lerma in the physiology of kainate receptors. In 2010, she was awarded a fellowship from the INA-Consolider Program and decided to join our group to carry out her thesis. She defended her Ph.D. thesis in September 2015 and obtained the maximal qualification. Thesis: Regulation of neuronal plasticity and responsiveness by the microRNA system. Publications with us: Fiorenza et al., Cereb Cortex 26(4):1619-33; Scandaglia et al., Sci Reports5:17470; Fiorenza & Barco, Neurobiol Learn Mem May 135:3-12. Other positions: Lab. Dr. Dorit Ron. University of California, San Francisco, San Francisco, USA; Institute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, USA.
• Deisy Guiretti studied Biology at the National University of Misiones, Argentina. She later investigated in the field of viral variability and phylogenia at the National Institute of Cancer in Rio de Janeiro and in the University of Edinburgh. She joined our lab in 2009 to carry out her thesis working on the etiology of Huntington’s disease. She defended her Ph.D. thesis in September 2015 and obtained the maximal qualification. Thesis: Lysine deacetylation and transcriptional dysregulation in neurological disorders: Huntington’s disease and the Rubinstein-Taiby syndrome. Publications with us: Valor et al., J Neurosci 33(25):10471-82; Guiretti D and Valor LM, Neuropharmacology 80:103-114; Guiretti et al., Neurobiol Dis 89:190-201; Hervas-Corpion et al., Sci Rep 8(1):9925; del Blanco et al., Cell Death Differ 26(11):2208-2222.
• Jose Viosca got his Master degree in Biochemistry in the Universidad de Valencia. He worked with us from October 2005 to May 2010 investigating the role of CREB and neuronal chromatin acetylation in learning and memory. He defended his Ph.D. thesis in May 2012 and obtained the maximal qualification. He also received the special distinction (Premio Extraordinario). He later moved to the laboratory of Cornelius Gross in EMBL-Monterotondo (Italy) for his first postdoctoral stay. Thesis: Common molecules in memory formation and congenital intellectual disability: a role for the Ras-ERK-CREB pathway in cognition. Publications with us: Viosca et al., J Neurosci 27(47): 12761; Viosca et al. Learn Mem16(3): 193; Viosca et al. Learn Mem 16(3): 198; Viosca et al. Genes Brain Behav 8(1):60; Viosca et al. Neurobiol Dis 37(1):186; Lopez-Atalaya et al. EMBO J 30(20):4287; Valor et al. Curr Pharm Des 19(28):5051-64. Other positions: Lab. Dr. Cornelius Gross. EMBL Monterotondo. Monterotondo, Italy.
• Eva Benito-Garagorri studied Biochemistry at the University of the Basque Country. She carried out her PhD in our laboratory working on the transcriptional response to neuronal activity and defended her Ph.D. thesis in June 2011, obtaining the maximal qualification. She is currently a postdoc at the laboratory of Andre Fischer in ENI-Gottingen (Germany). Thesis: A comparative transcriptomics approach for unveiling gene expression networks of activity-driven neuronal stimulation and plasticity. Publications with us: Viosca et al., J Neurosci 27(47): 12761; Viosca et al., Learn Mem 16(3): 198; Benito and Barco, Trends Neurosci 33(5): 230; Benito et al., J Neurosci 31(50): 18237; Gruart et al., J Neurosci 32(48): 17431; Lopez-Atalaya et al., Nucleic Acids Res. 41(17):8072-84; Yildirim F et al. Plos ONE 9(4); Benito and Barco. Mol Neurobiol. 289(47):32914-25; Scandaglia et al., Sci Reports 5:17470; Scandaglia et al. Cell Reports 21(1):47-59. Other positions: Lab. Dr. Andrè Fischer. DZNE, Gottingen, Germany. Scientific Coordinator for SourceData at EMBO, Heidelberg, Germany.
• Dragana Jancic studied Medicine and got her MD in the University of Belgrade (Serbia). She became a member of the Marie Curie Excellence Team in November 2004 and worked with us until July 2008. She defended her Ph.D. thesis in December 2008 and obtained the maximal qualification. She is currently a resident in the Psychiatry and Psychotherapy Department at the University Hospitals of Geneva (Switzerland). Thesis: Role of CREB-dependent transcription in the control of hippocampal neurons survival and plasticity. Publications with us: Barco et al. chapter in “Regulation of transcription by neuronal activity: To the nucleus and back” (Springer Sci Pub); Viosca et al. J Neurosci27(47): 12761; Lopez de Armentia et al. J Neurosci27(50): 13909; Viosca et al. Learn Mem 16(3): 193; Jancic et al. Cereb Cortex 19(11): 2535; Sanchis-Segura et al. Front Behav Neurosci3:30; Valor et al. Cell Death Differ 17(19): 1636. Other positions: Resident in Psychiatry and Psychotherapy, University Hospitals of Geneva. Geneva, Switzerland.

Other former lab members:

– Juan Medrano: Graduate student. He carried out his postgraduate studies from January 2017 to July 2020.
– Dr. Rafael Muñoz Viana: Posdoctoral fellow. He was part of the Team from June 2017 to December 2019.
– Marian Llinares: Technician. She was part of the Team from January 2018 to December 2019.
– Emanuele Zaccaria: Graduate student. He was part of the Team from June 2018 to June 2019.
– Alejandro Medrano: Graduate student. He carried out his Master thesis with us in 2015 and his postgraduate studies from January 2016 to July 2018.
– Nuria Cascales Pico: Technician. She was part of the Team from Julio 2016 to November 2017.
– Dr. Jose P. López-Atalaya: Senior postdoctoral fellow and Senior Researcher. He was part of the Team from 2006 to September 2015. He is now Principal Investigator at our Institute leading the «Cellular Plasticity and Neuropathology» group.
– Dr. Romana Tomasoni: Posdoctoral fellow and Visiting Researcher. She joined our lab from 2015 to 2016.
– Dr. Luis M. Valor: Posdoctoral fellow, Senior Researcher and finally co-PI. He was part of the Team from August 2007 to November 2015. He is now Principal Investigator at Hospital Universitario Puerta del Mar in Cadiz, Spain.
– Manuel Alcaraz Iborra: Graduate student. He was part of the Team from June 2012 to January 2014. He then went to the Psychology Department at University of Almeria (Spain) to conduct there his Ph.D. thesis.
– Victor Rovira: After finishing his thesis in the team of Emilio Geijo-Barrientes, he joined the Team from June to December 2013 before moving to , USA for a postdoc in the laboratory of Manuel Castro-Alamancos at Drexel University, Philadelphia, USA.
– Dr. Sven Parkel: Postdoctoral fellow. He was part of the Team from January 2012 to June 2013.
– Dr. Satomi Ito: Postdoctoral fellow. She was part of the Team from April 2009 to March 2013. She later worked at the Institute for Integrated Cell-material Sciences (iCeMS) in Kyoto University and the Okinawa Institute of Science anf Technology (OIST, Ichiro Maruyama’s lab), both in Japan.
– Pierrick Jego:Graduate student. He was part of the Team from October 2010 to September 2011.
– Francisca Almagro: Technician. She was part of the Team from November 2010 to July 2011.
– María Jiménez-Minchan: Technician. She was part of the Team from August 2008 to June 2011.
– Matías M Pulopulos: Graduate student. He was part of the Team from October 2009 to August 2010.
– Dr. Mikel Lopez de Armentia: Senior Researcher. He was part of the Team from November 2004 to August 2009.
– Miguel A. Andrés: Graduate student. He was part of the Team from July 2007 to December 2008.
– Marusa Arencibia: Secretary. She was our administrative officer from 2005 to 2008.
– Valentina Moscato: Graduate student. She worked as a member of the MC Excellence Team form September 2007 to April 2008.
– Petra Gromova: Graduate student. She was in the lab from November 2004 to July 2007.
– Ana Calvo: Graduate student. She was part of the Team from October 2005 to March 2006.

Visitors:

– María Consuelo López Gómez (undergraduate student, Master en Neurociencias UMH). Octubre 2019 – Enero 2021
– Pablo Castellano (undergraduate student, Master en Neurociencias UPO). May-October 2019
– Miguel Fuentes Ramos (undergraduate student, Universidad de Alicante, Alicante). Octubre 2017 – Septiembre 2018
– Marta Alaiz Noya (undergraduate student, Master en Neurociencias UMH). Octubre 2017 – Septiembre 2018
– Paula Mut (undergraduate student, Master en Neurociencias UMH). Octubre 2016 – Septiembre 2017
– Juan Marín (undergraduate student, Master en en Bioinformática y Biología Computacional de la Escuela Nacional de salud- ISCIII). May-October 2019
– Juan Medrano (undergraduate student, Universidad Jaume I, Castellón). July-September 2015
– Agnieszka Czechwska (undergraduate student, Warsaw University, Warsaw, Poland). July-August 2015
– Silvia Colmenero (undergraduate student, Universidad de Alicante, Alicante). may-September 2015
– Alejandro Medrano (undergraduate student, Universidad de Alicante, Alicante). June-October 2014
– Juan Manuel Agulló (undergraduate student, Universidad Autónoma de Barcelona, Barcelona). July-August 2014
– Adriana Magalska (postdoctoral fellow, Dr. Wilczynski’s lab, Nencki Institute, Warsaw, Poland). September to December 2013
– Marilyn Scandaglia (undergraduate student, University of Bologna, Bologna, Italy). April-October 2011
– Jorge Montesinos (undergraduate student, Universidad de Valencia, Valencia). Augus-September 2010
– Raffaela Tulino (undergraduate student, University of Sannio, Sannio, Italy). April-September 2010
– Alessandro Ciccarelli(PhD student, Dr. Giustetto’s lab, University of Torino, Torino, Italy). July 2009 to June 2010
– Paola Tognino (PhD student, Dr. Pizzorusso’s lab, CNR Pisa, Italy). February 2010
– Gianmarco di Mauro (undergraduate student, University of Bologna, Bologna, Italy). May-December 2009
– Vincent Prinz (postdoctoral fellow, Dr. Dirnagl’s lab, Charite Campus Mitte, Berlin, Germany). September 2009
– Jan-Albert Manenschijn (rotation student, Alicante). April 2009
– Eduardo Leiva (rotation PhD student, Alicante). February-March 2009
– Elena Carnero (undergraduate student, Universidad de Granada, Granada). Summer 2008
– Ferah Yildirim (PhD student, Dr. Dirnagl’s lab, Charite Campus Mitte, Berlin, Germany). July 2008
– Oskar Ortiz (PhD student, Dr. Moratalla’s lab, Instituto Cajal, Madrid). April 2008
– Carles Sanchis-Segura (Ramón y Cajal researcher, Universitat Jaume I, Castellon). October-November 2007
– Pankaj Sah (University Professor, University of Queensland, St. Lucia, Australia). September-October 2007
– Cristina Vicente (undergraduate student, Universidad Pablo de Olavide, Sevilla). Summer 2007
– Ignasi Sahun (PhD student, Dr. Dierssens lab,CRG, Barcelona). November 2005