Ómicas y Análisis Génico

BD FACS Discover S8 con Tecnología de Imagen BD CellView™ y Tecnología BD SpectralFX. La Tecnología de Imagen BD CellView™ amplía el poder del análisis y la clasificación celular al combinar datos de citometría de flujo con conocimientos espaciales y morfológicos. La Tecnología BD SpectralFX amplía el tamaño y la flexibilidad del panel con 78 detectores fluorescentes a través de cinco láseres (349nm; 405nm; 488nm; 561nm; 637nm) con filtros de paso de banda optimizados algorítmicamente.

FACS Aria III has four laser lines (Near Violet 375nm; Blue 488nm; Yellow/Green 561nm and Red 633nm) which allows covering the entire electromagnetic spectrum for applications of both endogenous fluorescence (fluorescent proteins such as gfp, cherry, tomato …), as well as antibodies conjugated to fluorophores. The fluidics and optical systems are precisely integrated to maximize signal detection. It is also equipped with a temperature control system between 4 and 37 degrees Celsius for both the sample and the cell collection tube.

BD FACSMelody is a last generation cell sorter which allows cell purification of both cell populations and single cell. It is equipped with three laser lines (Violet 405nm; Blue 488nm and Yellow /Green 561nm) for applications of both endogenous fluorescence (fluorescent proteins such as gfp, cherry, tomato …), as well as antibodies conjugated to fluorophores. It is also equipped with a temperature control system between 4 and 37 degrees Celsius for both the sample and the cell collection tube.

El software FlowJo™ es la plataforma líder para el análisis de citometría de flujo de una sola célula, que te ayuda a interpretar tus datos de manera rápida y efectiva con funciones accesibles para inmunofenotipado, análisis del ciclo celular, proliferación, estudios de cinética, comparación cuantitativa de poblaciones, análisis de alta dimensionalidad y más. Acceso al manual y tutoriales en este enlace: https://docs.flowjo.com/flowjo/

Chromium X es el instrumento más avanzado de 10X Genomics, compatible con todos los ensayos de célula única de la compañía. El instrumento Chromium X maneja de manera eficiente y confiable la partición celular, permitiéndote abordar fácilmente una amplia gama de proyectos, desde estudios piloto hasta proyectos a gran escala o en múltiples sitios. El completo portafolio de 10X Genomics permite el perfilado de muestras frescas, congeladas y fijadas (incluso FFPE) y la captura de múltiples niveles moleculares, incluyendo expresión génica, accesibilidad de la cromatina, proteínas, clonotipo inmune y cribado de perturbaciones por CRISPR.

It combines large partition numbers with a massively diverse barcode library to generate >100,000 barcode-containing partitions in a matter of minutes and allows a user to run any Chromium Solution, from genome to single cell analysis (Single cell gene expression, Single cell ATAC…). It allows working with different types of samples that range from high molecular weight (HMW) DNA, individual cells, cells labeled using Feature Barcoding technology, nuclei, nuclei treated with transposase, or cell Beads.

MERSCOPE es una plataforma de genómica espacial in situ de alta resolución que permite a los usuarios mapear transcritos de RNA en 1 cm² de tejido en una única rodaja de tejido, ofreciendo una visión de todo el tejido de hasta 1000 genes personalizados con resolución de célula individual.

The Mastercycler® pro S offers ultimate flexibility for your PCR applications. Equipped with a silver block, operate in various configurations, and use any plate, tube, or strip you want. All formats come with gradient SteadySlope® technology to ensure heating and cooling ramp rates remain consistent. With the vapo.protect technology, evaporation is kept to a minimum.

Applied Biosystems QuantStudio 3 Real-Time PCR System is a simple and affordable real-time PCR solution designed for users with all levels of experience. Get connected to your data easier than before with a real-time PCR systems that offers an interactive touch screen and the option to choose Web browser-based software enabled through the Thermo Fisher Cloud. It offers improved data accuracy and sensitivity for a broad range of applications, such as analyses of gene expression, microRNAs and noncoding RNAs, SNP genotyping, copy number variation, mutation detection….

The 2100 Bioanalyzer system is an established automated electrophoresis tool for the sample quality control of biomolecules. It provides highly precise analytical evaluation of various samples types in many workflows, including next generation sequencing (NGS), gene expression, biopharmaceutical, and gene editing research, and delivers objective assessment of sizing, quantitation, integrity and purity from DNA, RNA, and proteins. Minimal sample volumes are required for an accurate result, and the data may be exported in a many different formats for ease-of-use.

The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system optimized for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. The built-in cooling system (Bioruptor® Cooler) ensures high precision temperature control resulting in higher quality samples. Ideal for DNA shearing for Next-Generation-Sequencing (5 – 100 µl), Chromatin shearing (10 µl – 2 ml), RNA shearing, Protein extraction from tissues and cells, FFPE DNA extraction and Protein aggregation studies.

Countess II FL Automated Cell Counter is a benchtop assay platform equipped with state-of-the-art optics, full autofocus, and image analysis software for rapid assessment of cells in suspension. With three-channel flexibility—brightfield, researchers can count cells, evaluate apoptosis, and measure cell viability.

It allows working in an environment free of biological contamination for the preparation of delicate samples such as RNA, cDNA libraries….

The computing platform provides researchers at Instituto de Neurociencias and external organizations with high-performance computing resources for next-generation sequencing (NGS) data processing and analysis.

Server main features and configuration:

• CPU: 4 Cores Intel(R) Xeon(R) CPU E7-8870 v4 @ 2.10GHz -> 80 cores with HyperThreading [160 Threads] with 200MB cache storage L3.
• RAM: 2TB 2400MHz -> Registered 1600MHz.
• SSD: 4TB in parity RAID5 (4 SSD x 1TB each).

Dedicated NAS:

• HDD: 22.6TB available (8 6TB HDD configured in parity RAID 6 plus 1 hot spare disk).
• CPU AMD GX-420MC SOC @ 2.0 GHz -> 4 cores, [4 Threads].
• RAM: 4GB.