English
Líneas de investigación
La organización de las células en tejidos en todos los animales depende de la membrana basal (MB), un polímero planar de proteínas de matriz extracelular que subyace epitelios y rodea órganos y tejido nervioso. Comprender la arquitectura de los tejidos y sus determinantes celulares y moleculares es esencial para descifrar los mecanismos del desarrollo normal del sistema nervioso y su fisiología alterada asociada a enfermedades y envejecimiento. En nuestro laboratorio utilizamos la mosca de la fruta Drosophila melanogaster para estudiar la secreción y el ensamblaje de la MB. Aprovechando las sofisticadas herramientas genéticas disponibles en Drosophila, y en combinación con técnicas de imagen avanzada, investigamos la biogénesis de los componentes de la MB, su ensamblaje en polímeros normales o fibróticos, y su papel en la morfogénesis del sistema nervioso, señalización intercelular, respuesta inmune, regeneración y progresión tumoral.
Un aspecto particularmente interesante de la biogénesis de la matriz extracelular es su tráfico a través de la vía secretora. Las enormes dimensiones del colágeno y otras proteínas de la matriz las hacen propensas a agregar fuera de la célula de manera dependiente de la edad y desafían los modelos actuales de cómo funciona la vía de secreción. Por ello, estamos examinando la organización altamente especializada de la vía secretora en dendritas neuronales en los llamados “puestos avanzados del Golgi”: fragmentos del Golgi que carecen de la organización altamente polarizada típica de esta organela. Entender la formación de “puestos avanzados” puede ayudar a descifrar los mecanismos de la fragmentación del Golgi, notablemente incrementada en enfermedades neurodegenerativas.
Publicaciones relevantes
- Convergence of secretory, endosomal, and autophagic routes in trans-Golgi-associated lysosomes. Zhou, L., Xue, X., Yang, K., Feng, Z., Liu, M., Pastor-Pareja, J.C. J Cell Biol. 2023 222(1):e202203045 https://doi.org/10.1083/jcb.202203045
- FGF signaling promotes spreading of fat body precursors necessary for adult adipogenesis in Drosophila. Lei Y , Huang Y, Yang K, Cao X, Song Y, Martín-Blanco E, Pastor-Pareja JC PLoS Biol. 2023 21: e3002050 https://doi.org/10.1371/journal.pbio.3002050
- ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriers Yang K, Liu M, Feng Z, Rojas M, Zhou L, Ke H, Pastor-Pareja JC Cell Rep. 2021 36:109707 https://doi.org/10.1016/j.celrep.2021.109707
- Atypical laminin spots and pull-generated microtubule-actin projections mediate Drosophila wing adhesion Sun T, Song Y, Teng D, Chen Y, Dai J, Ma M, Zhang W, Pastor-Pareja JC Cell Rep. 2021 36:109667 https://doi.org/10.1016/j.celrep.2021.109667
- Atypical basement membranes and basement membrane diversity – what is normal anyway?. Pastor-Pareja JC. J Cell Sci. 2020 133: jcs241794 - Review https://doi.org/10.1242/jcs.241794
- Spectraplakin Shot maintains perinuclear microtubule organization in Drosophila polyploid cells Sun T, Song Y, Dai J, Mao D, Ma M, Ni J-Q, Liang X, Pastor-Pareja JC Dev Cell 2019 49:731-747.e7 https://doi.org/10.1016/j.devcel.2019.03.027
- Tango1 spatially organizes ER exit sites to control ER export Liu M, Feng Z, Ke H, Liu Y, Sun T, Dai J, Cui W, Pastor-Pareja JC J Cell Biol 2017 216:1035-1059 https://doi.org/10.1083/jcb.201611088
- Basement membrane manipulation in Drosophila wing discs affects Dpp retention but not growth mechanoregulation Ma M, Cao, Dai J, Pastor-Pareja JC Dev Cell 2017 42:97-106.e4 https://doi.org/10.1016/j.devcel.2017.06.004
- Inter-adipocyte Adhesion and Signaling by Collagen IV Intercellular Concentrations in Drosophila. Dai J, Ma M, Feng Z, Pastor-Pareja JC. Curr Biol. 2017 27: 2729-2740.e4 https://doi.org/10.1016/j.cub.2017.08.002
- Plasma membrane overgrowth causes fibrotic collagen accumulation and immune activation in Drosophila adipocytes. Zang Y§, Wan M§, Liu M, Ke H, Ma S, Liu LP, Ni JQ, Pastor-Pareja JC. eLIFE 2015 4: e07187 https://doi.org/10.7554/eLife.07187
Investigador principal
Investigador doctor
Predoctoral / Apoyo investigación
Técnico
- Convergence of secretory, endosomal, and autophagic routes in trans-Golgi-associated lysosomes. Zhou, L., Xue, X., Yang, K., Feng, Z., Liu, M., Pastor-Pareja, J.C. J Cell Biol. 2023 222(1):e202203045 https://doi.org/10.1083/jcb.202203045
- FGF signaling promotes spreading of fat body precursors necessary for adult adipogenesis in Drosophila. Lei Y , Huang Y, Yang K, Cao X, Song Y, Martín-Blanco E, Pastor-Pareja JC PLoS Biol. 2023 21: e3002050 https://doi.org/10.1371/journal.pbio.3002050
- Intrinsic and damage-induced JAK/STAT signaling regulate developmental timing by the Drosophila prothoracic gland. Cao X, Rojas M, Pastor-Pareja JC. Dis Mod Mech. 2022 15: dmm049160 https://doi.org/10.1242/dmm.049160
- ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriers Yang K, Liu M, Feng Z, Rojas M, Zhou L, Ke H, Pastor-Pareja JC Cell Rep. 2021 36:109707 https://doi.org/10.1016/j.celrep.2021.109707
- Atypical laminin spots and pull-generated microtubule-actin projections mediate Drosophila wing adhesion Sun T, Song Y, Teng D, Chen Y, Dai J, Ma M, Zhang W, Pastor-Pareja JC Cell Rep. 2021 36:109667 https://doi.org/10.1016/j.celrep.2021.109667
- Tales of the ER-Golgi Frontier: Drosophila-Centric Considerations on Tango1 Function. Feng Z, Yang K, Pastor-Pareja JC. Front Cell Dev Biol. 2021 8: 619022 - Review https://doi.org/10.3389/fcell.2020.619022
- Katanin p60-like 1 sculpts the cytoskeleton in mechanosensory cilia Sun L, Cui L, Liu Z, Wang Q, Xue Z, Wu M, Sun T, Mao D, Ni J-Q, Pastor-Pareja JC, Liang X. J Cell Biol. 2021 220: e202004184 https://doi.org/10.1083/jcb.202004184
- Atypical basement membranes and basement membrane diversity – what is normal anyway?. Pastor-Pareja JC. J Cell Sci. 2020 133: jcs241794 - Review https://doi.org/10.1242/jcs.241794
- Premature termination codon readthrough in Drosophila varies in a developmental and tissue-specific manner. Chen Y, sun T, bi Z, Ni J-Q, Pastor-Pareja JC, Javid B. Sci Rep. 2020 10: 8485 https://doi.org/10.1038/s41598-020-65348-8
- Spectraplakin Shot maintains perinuclear microtubule organization in Drosophila polyploid cells Sun T, Song Y, Dai J, Mao D, Ma M, Ni J-Q, Liang X, Pastor-Pareja JC Dev Cell 2019 49:731-747.e7 https://doi.org/10.1016/j.devcel.2019.03.027
- Dissection of Nidogen function in Drosophila reveals tissue-specific mechanisms of basement membrane assembly. Dai J , Estrada B, Jacobs S, Sánchez-Sánchez-B , Tang J, Ma M, Magadán-Corpas P, Pastor-Pareja JC , Martín-Bermudo MD. PLoS Genet. 2018 14: e1007483 https://doi.org/10.1371/journal.pgen.1007483
- Collagen secretion screening in Drosophila supports a common secretory machinery and multiple Rab requirements. Ke H, Feng Z, Liu M, Sun T, Dai J, Ma M, Liu L-P, Ni J-Q, Pastor-Pareja JC. J Genet Genom. 2018 45: 299-313 https://doi.org/10.1016/j.jgg.2018.05.002
- The Drosophila Hox gene Ultrabithorax controls appendage shape by regulating extracellular matrix dynamics. de las Heras J, Garcia-Cortés C, Foronda D, Pastor-Pareja JC, Shashidhara LS, Sánchez-Herrero E. Development. 2018 145: dev161844 https://doi.org/10.1242/dev.161844
- Tango1 spatially organizes ER exit sites to control ER export Liu M, Feng Z, Ke H, Liu Y, Sun T, Dai J, Cui W, Pastor-Pareja JC J Cell Biol 2017 216:1035-1059 https://doi.org/10.1083/jcb.201611088
- Basement membrane manipulation in Drosophila wing discs affects Dpp retention but not growth mechanoregulation Ma M, Cao, Dai J, Pastor-Pareja JC Dev Cell 2017 42:97-106.e4 https://doi.org/10.1016/j.devcel.2017.06.004
- Inter-adipocyte Adhesion and Signaling by Collagen IV Intercellular Concentrations in Drosophila. Dai J, Ma M, Feng Z, Pastor-Pareja JC. Curr Biol. 2017 27: 2729-2740.e4 https://doi.org/10.1016/j.cub.2017.08.002
- Extracellular chloride signals Collagen IV network assembly to form basement membranes outside cells. Cummings CF, Pedchenko V, Brown KL, Colon S, Rafi M, Stothers C, Ero-Tolliver IA, Jones-Paris C, Pokydeshava E, Liu M, Pastor-Pareja JC, McCall AS, Vanacore R, Gautam Bhave G, Santoro S, Blackwell T, Zent R, Pozzi A, Hudson BG. J Cell Biol. 2016 213 (4): 479–494 https://doi.org/10.1083/jcb.201510065
- Localized JNK signaling regulates organ size during development. Willsey HR, Zheng X, Pastor-Pareja JC, Willsey AJ, Beachy PA, Xu T. eLIFE 2016 5: e11491 https://doi.org/10.7554/eLife.11491
- Plasma membrane overgrowth causes fibrotic collagen accumulation and immune activation in Drosophila adipocytes. Zang Y§, Wan M§, Liu M, Ke H, Ma S, Liu LP, Ni JQ, Pastor-Pareja JC. eLIFE 2015 4: e07187 https://doi.org/10.7554/eLife.07187
- Toll pathway modulates TNF-induced JNK-dependent cell death in Drosophila. Wu C, Chen C, Dai JL, Zhang F, Chen Y, Li W, Pastor-Pareja JC, Xue L. Open Biol. 2015 5: 140717 https://doi.org/10.1098/rsob.140171
