Molecular Biology & Microbiology

Spectrophotometers

  • Infinite M Nano plate reader (room 245)
    Versatile plate reader with monochromator ideal for ELISA assays, protein quantification assays (Bradford, BCA, Lowry) and Cell proliferation/viability assays (MTT, XTT, etc)..

User’s manual-Software   

User’s manual

  • Biochrom Ultrospec 2100 pro (room 023)
    Stand alone UV-Visible cuvette spectrophotometer to measure absorbance and/or concentration of nucleic acids and proteins, among others.

User’s manual

  • Thermo Nanodrop ND 1000 (room 245)
    Low volume (1-2µl) absorbance reader (220-750nm) ideal for nucleic acid and protein quantification.

User’s manual

  • Thermo Nanodrop ONE (room “omics”)
    Low volume (1-2µl) absorbance reader (220-750nm) ideal for nucleic acid and protein quantification.

User’s manual

Ovens/Incubators

  • Binder refrigerated incubator KB 2040 (room 113)
    Climatic chamber (from -5ºC to 100ºC, up to 247L) 

User’s manual

  • CIC Controltecnica SH Maxi  (room 119)
    Orbital shaker incubator (from RT + 5Cº to 60ºC and 30 to 300 rpm) with capacity for 5 x 500 ml. 

User’s manual

  • ING Climas CIR-S 250/HR (room 119)
    High capacity fridge/incubator (up to 250L, from 0Cº to 60ºC)

User’s manual

  • New Brunswick Innova 44 (x2) (room 113,119)
    High capacity incubator (from RT + 5Cº to 60ºC and 25 to 250 rpm)

User’s manual

  • Selecta Incudigit oven (x2) (room 23,205)
    Regular culture /desiccation oven. 

User’s manual

  • Thermo Orbital shaker (x2) (room 119,205)
    Orbital shaker incubator (from RT + 5Cº to 80ºC and 25 to 525 rpm) with capacity for up to six 2L flasks.

  User’s manual

Imaging

  • Alpha Innotech Alphaimager 2200 (x2) (room 023)
    Gel UV/White Documentation system 

User’s manual

  • Amersham Imager 680 (room 245)
    High-sensitivity digital Blot/Gel chemiluminescense and fluorescense imager (16-bit) 

User’s manual

  • iBright CL15000 (room 245)
    High-sensitivity digital Blot/Gel chemiluminescense and fluorescense imager (24-bit)

User’s manual

  • GE Image scanner III (room 245)
    Two-dimensional electrophoresis gel and blot scanner (up to 600 dpi) 

User’s manual

  • Li-Cor Odyssey (room 245)
    High-sensitivity Infrared and chemiluminescense imager (22-bit) 

User’s manual

Sonifiers

  • Branson Sonifier SFX550 (room 023)
    Sonifier for cell diruption, homogenization and/or emulsification through cavitation (550W) equipped with low and high-volume tips (from 0.2 to 1000ml)

  User’s manual

Electrophoresis

  • Bio-Rad Pulse Field Electrophoresys System CHEF-DR II (room “omics”): Pulse Field Electrophoresis (120º) for chromosome-size DNA molecules (up to 250V and 500mA).  User’s manual
  • GE Ettan IPGphor Isoelectric Focusing System (room “omics”): Two-dimensions (up to 12 strip holders and 8000 V)  User’s manual
  • GE MiniVe Electrophoresys System room “omics”): Vertical Electrophoresis system (tank vol. 1.7L)  User’s manual

Another equipments

  • Speed-Vac Eppendorf (room 023)
    Concentrator with PTFE filter membrane and condensation trap.

User’s manual

  • Bio-Rad Gene Pulser II Electroporation system (room 023)
    Cell Electroporation system (50-2500V) for cell transformation.

User’s manual

  • IKA Orbital shaker KS 260 (room 113)
    Compact orbital shaker (50-500 rpm). 

User’s manual

  • Speed-Vac DNA mini (room 023)
    Centrifugal Vacuum Evaporator/Concentrator (Temp range from 30 to 60ºC, and up to 12 mbar vaccum and 1800 rpm centrifugation)

  User’s manual

  • Precisa Balance XT120A (room 023)
    Precision balance (up to 120g, readability 0.1mg) 

User’s manual