The Cell Culture Unit is the Insituto de Neurociencias Service that provides researchers the environment for getting healthy viable cell cultures. This Service is composed by three different and spatially separated Areas in order to carry out different types of cell cultures: Cell Lines, Primary Cultures and Organotipic Cultures.
Each of these facilities are well equipped with class I and/or class II laminar flow cabinets, incubators, inverted phase contrast and fluorescence microscopes and all the material necessary to perform specialized cell culture techniques. Bio-safety level 2 areas are included to work with high risk material (human samples, virus).The Unit also have available a new generation system for real-time quantitative live-cells analysis.
- Technical advice for culturing a range of different cell types.
- Training and technical assistance in cell culture protocols and in the use of all the devices and equipment provided in the Facilities.
- Mycoplasma analysis for all the cells to guarantee they are free of contaminants.
- Frozen cell stocks conservation in liquid nitrogen.
- Equipment and devices maintenance.
- Media and pre-culture material preparation and sterilization.
Cryopreservation and water purification system of the Cell Culture units:
Listado de servicios ofertados
General operational considerations of the Cell Culture Unit:
- 1. Cell Culture Areas are highly specialized zones so people crowds will not be allowed.
- 2. The Principal Investigator is ultimately responsible for the correct use of the Facility’s equipment by any member of his/her team.
- 3. All users must contact with the Unit Supervisor to obtain the authorization in order to work in the Unit.
- 4. It is mandatory for new users to receive the corresponding training before working in the Unit. Furthermore, during the first session they should be supervised by an experimented user or the Facility’s personnel.
- 5. Users must read and be aware of the basic instructions for the correct use of each equipment he/she manipulates, as well as for the rules applicable in each case.
- 6. Biosafety cabinets and equipment will be always booked through the established system.
- 7. If a booked session is not going to be used must be cancel.
- 8. Any user booking a slot is responsible for the equipment he/she uses, and has to kept clean and tidy the area after finishing.
- 9. If you have any doubt regarding the use of any equipment ask for help to the qualified personnel.
- 10. General communication, questions and bookings modifications will be preferably stablished by e-mail to: firstname.lastname@example.org
Cell Culture Unit is distributed in three main areas according to the culture type that will perform:
Room 237. Primary Cultures
Room 129. Cell Lines
- Preculture area (Rosa García)
- Fluorescence microscope dark room
- Culture room 1. Long term cultures and Live-cell image acquisition (Incucyte)
- Culture room 2. Primary cultures preparation
- Culture room 3. Primary cultures preparation
- Culture room 4. Biohazardous cultures (human samples, virus infections)
Room 111. Organotipic Cultures
- Culture room 1. Biohazardous cultures (human samples, virus infections) and fluorescence microscope
- Culture room 2. Quarantine area
- Culture room 3. Stem cell cultures
- Culture room 4. Cell lines maintenance
- Preculture area (Sara Carratalá)
Cryopreservation Area (shed in the loading dock)
- Preculture area
- Culture room 1. Organotipic cultures preparation
- Culture room 2. Biohazardous cultures (human samples, virus infections)
- Manual filling containers (three)
- Automatic vapor phase container