Líneas de investigación
Brain function generates cognition, thought and adaptive behaviors through coordinated actions of circuits that are hard-wired during development with others that retain remarkable plasticity into adulthood. A fundamental question is how experiences shape these neural circuits, so the individual learns and interacts adequately with its environment. Over postnatal stages, most remodeling involves formation, strengthening or elimination of synapses to build, maintain or reshape neural assemblies. Synaptic remodeling occurs throughout life but is maximal during “so-called” critical periods—a stage of postnatal development when synapses have a high potential for plasticity and massive formation and elimination of synapses refines initially redundant circuitry. Yet this plasticity potential needs to be “tamed” so the correct synaptic partners are specified during postnatal circuit refinements and to support precise learning and cognitive-guided behaviors. Understanding how this is achieved is one major goal of our lab.
A central research theme stems from our discovery of a class of NMDA receptors, defined by the presence of non-conventional GluN3A subunits, that work as gate-keepers of experience-dependent plasticity and synaptic refinements (Nature Rev Neuroscience 2016). Transient waves of GluN3A expression are typical of primary or sensory cortical areas and guide the hard-wiring of sensory circuits. By contrast, adult expression is retained into adulthood in less-differentiated association and trans-modal cortical areas, high-order thalamic nucleus and regions engaged in emotional control (Cerebral Cortex 2021). In the last years, we have generated a collection of mouse genetic tools to map cellular populations, circuits and behaviours that rely on GluN3A plasticity and understand its role in functional integration.
Other areas of investigation include:
1) Targeting circuit plasticity and cognition: Neurons rely on translational control to direct persistent modifications to selected synapses, but the mechanisms affording synapse and temporal specificity had been elusive. We have discovered neuronal GIT1/mTORC1 complexes that nucleate protein synthesis at synapses and whose assembly is negatively regulated by GluN3A expression, imposing limits on memory capacity. Regulated interactions between GluN3A and GIT1 determine which memories will be persistently stored, opening an entry point for modulating cognition (eLife 2021).
2) Identifying plasticity niches in non-neuronal cells
- Control of protein synthesis and memory by GluN3A-NMDA receptors through inhibition of GIT1/mTORC1 assembly María J Conde-Dusman,Partha N Dey, Óscar Elía-Zudaire, Luis G Rabaneda, Carmen García-Lira, Teddy Grand, Victor Briz, Eric R Velasco, Raül Andero, Sergio Niñerola, Angel Barco, Pierre Paoletti, John F Wesseling, Fabrizio Gardoni, Steven J Tavalin, Isabel Perez-Otaño eLife 2021 10:e71575 (Nov 17, 2021) https://doi.org/10.7554/eLife.71575
- Temporal dynamics and neuronal specificity of Grin3a expression in the mouse forebrain. Murillo, A.,Navarro, A.I., Puelles, E., Zhang, Y., Petros, T., Pérez-Otaño, I Cerebral Cortex 2020 31 (4): 1914–1926 http://doi.org/10.1093/cercor/bhaa330
- RNAi-based GluN3A silencing resolves disease phenotypes in HD models Marco, S.,Murillo, A. Pérez-Otaño, I., Molecular Therapy 2018 26(8): 1965- 1972
- Emerging roles of non-conventional NMDA receptors in synapse development and disease. Pérez-Otaño I.,Larsen RS, Wesseling JF. Nature Reviews in Neuroscience 2016 17(10): 623- 35 10.1038/nrn.2016.92
- GluN3A expression restricts maturation of inactive synapses via direct binding to GIT1. Fiuza M.,González-González I, Pérez-Otaño I. Proc Natl Acad Sci USA 2013 110(51): 20807- 12 http://doi.org/10.1073/pnas.1312211110