Microscopy Facility

• Training and technical assistance for the use of all the devices and equipment provided in the Facility.
• Coaching/guidance for experimental design.
• Training and assistance in the processing and analysis of images using the Facility’s commercial and freely available software.
• Guidelines elaboration and assistance in the writing of materials and methods for publications.
• Maintenance and improvement of equipment and the entire infrastructure of the unit.
• Guidance in the acquisition of microscopy equipment.
• Equipment and device maintenance.
• Workshop organization and displays related to image acquisition, processing and analysis, as well as new technologies.
• Preparation of documentation for the acquisition of new equipment for the Microscopy Service.
• Participation in outreach activities.

Training Request

Leica service request

View the document “Guidelines of the Microscopy Facility” found in the ARCHIVES section for a more complete description of the equipment.

Multiphoton Microscopes (Lab 020)

Confocal Microscopes

Light-sheet Microscopes

Widefield Microscopes

Leica MZFLIII fluorescence stereoscope

Leica MZFLIII fluorescence stereoscope (Lab 021)

Workstations

HIVE high-performance workstation
It is a high-end centralized workstation for image processing and high-performance/GPU computing, optimal for demanding applications and multi-user configurations. It contains ZEISS arivis Pro, Huygens Professional, FIJI, QuPath, Zen Blue (Zeiss) and Las X (Leica) software.

Superresolution Workstation
Zen: https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html
Equipped with ZEN Black, ZEN Blue, Huygens Professional, Leica LAS X Core Widefield, Imaris Viewer, ImageJ/Fiji.

Workstation Lightsheet
Equipped with: ZEN Black, ZEN Blue 3.3 Lite, Leica LAS X Core Widefield, Imaris Viewer , ImageJ/Fiji, Huygens Professional.

Imaris I
Equipped with: Imaris, AutoQuant X3, Huygens Professional, ImageJ/Fiji, Olympus FV10-ASW Viewer, Leica LAS X Core, ZEN Blue 3.3 Lite de Zeiss, Terrastitcher ImspectorPro de LaVisionBiotec.

Imaris II
Equipped with: Imaris, Imaris Stitcher, ImageJ/Fiji 64 bit, Olympus FV10-ASW Viewer, Leica LAS X Core, ZEN Blue 3.3 Lite de Zeiss, ClearMap, Huygens Core HRM.

Workstation
ImageJ/Fiji, Neurolúcida Explorer, Olympus FV10ASW Viewer, ZEN Blue 3.3 Lite y Leica LAS X Core.

Servidor NAS

For temporary storage of files generated on the facility equipment that require further processing with Imaging Facility scientific software.

View the document “Guidelines of the Microscopy Facility” found in the ARCHIVES section for a more complete description of the facility operation.

General operational considerations of the Microscopy Facility:

1. General communication, questions, appointment inquiries, and training requests will be established by email to: microscopia@umh.es.

2. The Principal Investigator is ultimately responsible for the correct use of the Facility’s equipment by any member of his/her team.

3. All research personnel affiliated with the IN and those visiting researchers who are going to stay at the IN for more than 6 months will have the status of Microscopy Facility user (with the exception of those who can prove that they are experts in microscopy techniques).

4. Master’s or degree students may use the equipment only under the company of a supervisor.

5. It is the obligation of each user to know the operating rules of the Microscopy Service, as well as the specific instructions for handling each equipment used. These are included in the document “Imaging Facility Guidelines”.

6. It is mandatory for new users to receive the corresponding training by the Facility’s personnel before using any equipment. Moreover, during the first sessions, they should be supervised by the Facility’s personnel or an experienced user.

7. Training requests will be made through the training request form located in the “Services” section of our website, once the new user:

   – Has their own prepared samples and sufficient specific knowledge about them.
   – Has clear specific questions to answer with the facility equipment.
   – Is going to use the equipment imminently.

8. The reserved user is responsible for the state of the equipment during that period.

9. The last booked user of the day is responsible for turning off the microscope and all its components.

10. The working area should be kept clean, particularly after finalizing the session.

11. If you have uncertainties regarding the use of any equipment, no matter how simple may seem, ask for help from the personnel without any hesitation.

12. It is forbidden to browse Internet, software installation/deletion or the use of external storage devices in any Facility’s computer.

13. It is forbidden to store data on the computers of the facility. Users will be responsible for transferring their images once acquired or processes, as well as removing them from their user folder. An automatic file deletion instruction is programmed in all Microscopy Facility equipment. Files older than 30 days since their acquisition will be deleted from the computer and cannot be restored.

14. Users should request their subscription to the mailing list in.microscopia@listas.umh.es through the website https://listas.umh.es/sympa/info/in.microscopia. Communications regarding the Imaging Facility will be made through this mailing list.

15. Microscopes and workstations will be always booked through the web: http://in.umh-csic.es/ > Acceso usuarios > Reservas. All the equipment and software offered by the Imaging Facility begin with the letter “SM”.

16. Users are required to start and end their reservation on time so as not to interfere with the work of other colleagues.

17. Reservations not used during the first 10 minutes will be considered deserted.

18. Although the reservation system allows cancellations of bookings at any time, cancellations on the current day are not allowed and will be penalized if they are not duly justified as follows:

– Users who cancel an appointment reserved electronically on the current day will be penalized with two weeks without access to the service equipment.

– Users who do not use or release a booking via telematics will not be able to use the service equipment for the following four weeks.

– In case of recidivism by the user, use of the Service will be prohibited for three months

Reservations for all the facility equipment will be made through the intranet of the Institute of Neurosciences

The Microscopy Facility is free for the Instituto de Neurociencias members. If you do not belong to our Institute but would like to collaborate with one of our research teams to use our knowledge and technology in any of your projects, please contact us by email: microscopia@umh.es

View TECHNOLOGICAL OFFER

To consult our rates for external users, please access the following links of the Technical Support Service for Teaching and Research (Satdi) of the UMH:

“Fluorescent and Confocal Microscopy”:

https://satdi.umh.es/instrumentacioncientifica/servicios_ofertados/microscopia-confocal/

How to use the Satdi service:

https://satdi.umh.es/instrumentacioncientifica/

How to make a service use request:

https://satdi.umh.es/instrumentacioncientifica/formularios/formulario-peticion/

The Microscopy Facility is located on the ground floor, south corner of the IN and organized as follows:

Room 018

• Office 1: Giovanna Expósito
• Office 2: Almudena Íñigo
• Imaris I
• Imaris II
• Workstation

Room 019

• Inverted Microscope Leica Thunder Imager
• Slide scanner Zeiss AxioScan 7

Room 020

• Vertical Multiphoton Microscope Leica SP5
• Inverted Multiphoton Microscope Leica SP2
• Light-sheet Ultramicroscope II LaVisionBiotec

Room 021

• Vertical Confocal Microscope Leica SPEII
• Inverted Confocal Microscope Olympus FV1200
• Inverted Super-resolution Confocal Microscope Zeiss LSM 880-Airyscan Elyra PS.1
• Workstation Super-resolution
• Light-sheetZ.1 Microscope Zeiss
• Workstation Light-sheet in vivo
• Neurolucida System
• Leica MZFLIII fluorescence stereoscope
• Vertical Leica DM5000B Microscope
• Confocal spinning disk Dragonfly

Software packages available at the Facility:

• Imaris (Bitplane): software for visualization, processing, analysis and multichannel image export in 2D, 3D and 4D.
  http://www.bitplane.com/
• Zeiss arivis Pro: software for visualization, processing, analysis and multichannel image export in 2D, 3D and 4D.https://www.arivis.com/products/pro
• Neurolucida: http://www.mbfbioscience.com/neurolucida
• Strereo Investigator: https://www.mbfbioscience.com/stereo-investigator
• Huygens Profesional: https://svi.nl/Huygens-Software
• AutoQuant X3: deconvolution software.http://www.mediacy.com/autoquantx

Free softwares for image visualization and analysis:

• ImageJ: free software for processing and analysis of multidimensional images.https://imagej.nih.gov/ij/
• Fiji: same as ImageJ, which includes a complete processing package for scientific image analysis.https://imagej.net/Fiji
• ClearMap: free software crated for the image analysis of iDISCO+ samples. It allows the detection of 3D objects and brain nuclei localization.https://idisco.info/clearmap-2/
• Zeiss: https://www.zeiss.com/microscopy/int/products/microscope-software/zen-lite.html
• Leica: https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/downloads/

Tutorials:

• Imaris: http://www.bitplane.com/learning
• Zeiss arivis Pro: https://kb.arivis.com/arivis-vision4d-video-tutorials-compendium
• ImageJ/Fiji: http://imagej.net/Category:Tutorials#Tutorials_for_new_users
• Neurolucida: http://www.mbfbioscience.com/webinars
• Stereology: https://www.stereology.info
• Optic microscopy: http://zeiss-campus.magnet.fsu.edu/
• Light-sheet microscopy: https://www.youtube.com/watch?v=vKHQudCKVGc
• Huygens Professional: https://svi.nl/tiki-index.php?page=IntroductionMovies
• LAS X Thunder: https://youtu.be/36HqrFyy0fQ
• LAS X Navigator: https://youtu.be/iqMgOmjslf8
• Bioimage analysis: https://bioimagebook.github.io/README.html

Tissue clearing techniques:

• https://idisco.info/
• https://www.lavisionbiotec.com/products/UltraMicroscope/scanner-options.html
• http://clarityresourcecenter.org/
• https://transparent-human-embryo.com/

Ethics in digital image processing:

• https://www.ncbi.nlm.nih.gov/pubmed/?term=Cromey+digital+imaging+ethics

Spectrum visualization:

• https://www.fpbase.org/spectra/
• https://www.thermofisher.com/order/fluorescence-spectraviewer#!/

» Microscopia de alta resolución automatizada y apoyo a manejo de datos de imagen de alta densidad para Unidad de Imagen EQC2019-006023-P. FEDER/Ministerio de Ciencia e Innovación – Agencia Estatal de Investigación.

» Actualización del Servicio de Imagen celular ECQ_2018_004690_P. FEDER/Ministerio de Ciencia e Innovación Agencia Estatal de Investigación, cofinanciado con el proyecto Severo Ochoa SEV-2017-0732.

» Actualización de Servicio de Imagen Celular CSIC15-EE-3246 FEDER/Ministerio de Economía, Industria y Competitividad, cofinanciado por el proyecto Severo Ochoa SEV-2013-0317.

Scientific Director:
Eloísa Herrera
e.herrera@umh.es »
+34 965 91 92 31

Technical Manager:
Giovanna Expósito Romero

gexposito@umh.es »
+34 965 91 92 56

Technical:
Almudena Iñigo Portugues
ainigo@umh.es »
+34 965 91 92 56

General communication, questions, appointment inquiries, and training requests will be set up by email to: microscopia@umh.es

Microscopy Facility
Instituto de Neurociencias
Universidad Miguel Hernández – CSIC
Campus de San Juan
Sant Joan d’Alacant
Alicante | España

Tel. + 34 965 23 37 00
(ext. service 9256 / 9266)
Fax + 34 965 91 95 61