Resp. scientific /technical:
Berta López
–
Technical staff
:
Rosa García
1. For the use of the equipment reservation through the intranet of the IN website is necessary: intranet equipment reservation
2. For the common isofluorane anesthesia equipment, there is a maximum of 16 hours allocated per research group at restricted times (9.00 to 18.00).
3. Outside the restricted use schedule, there is no restriction in the reservation of slots although these must be always annotated.
4. Canceling bookings prior to one day can be done through the web page. Cancellations the same day that the equipment is booked must be done by email to in.personal in order to release the slot (mail: in.personal)
5. Working area should be kept clean, particularly after finalizing the session.
6. Any questions, contact the person in charge of the service.
7. Use of the anesthesia equipment:
-Isofluorane tanks must be filled in completely (up to the upper line) once the procedures are finished and every time it is used.
-Users have to fill in the “Isofluorane Use record” with the data required (name, group and time of use) everytime the equipment is used.
-Isofluorane refill records needs to be updated.
-Close O2 tank after using the equipment.
Contact Jesus Campos (jesus.campos@umh.es) and Berta Lópz Sánchez-Laorden (berta.lopez@umh.es) when the O2 tank is running out.
This facility is located in room 105 (First floor).
Common surgery equipment:
- Isofluorane and oxygen anesthesia system
- Anesthesia induction chamber
- Stereomicroscope and light source
- Heating blancket
- Minifugue for eppendorf tubes
- Waterbath
- Heating plate
- Fluorescence Estereomicroscope Leica MZ16FA (GFP and DsRed filters)
Electroporator in vivo Cuy21Edit
CUY21EDIT(Electroporator) can introduce DNAs, siRNAs, drugs, into tissues
including brain or embryos.
• General characteristics: -Voltage 1~500V -Pulse time
0.1~999.9ms -Pulse Interval 0.1~999.9ms -Pulse number 1~99 time(s) -Impedance
measurement ~18kO -Effective voltage 1~500V -Effective current 0.01~4.00
• Applications: In vivo, in utero, ex utero, in ovo, ex ovo,
ex vivo electroporation.
Vevo 3100 micro Ultrasound Imaging System
Ultrasonic imaging system adapted for use in small animals including mouse and
zebrafish. This system makes possible a high-resolution anatomical
visualization to perform cardiovascular, renal, reproductive system,
oncological or embryonic development studies in real time.
• General characteristics. Equipment includes:
-Microinjector
-Isofluorane anesthesia system
-MX201 transducer: 10-22 MHz, Centre Transmit: 15 MHz, Axial Resolution: 100
µm
-MX550D transducer: Centre Transmit: 40 MHz, Axial Resolution: 40 µm
• Applications:
Developmental biology:
-Gestation monitorization and determination of embryonic stage
-Analysis of placental structure and function
-Monitoring of organ development in the embryo
-Injection of viruses, cells or drugs into intra-uterine embryos: in the
brain, eye, spinal cord or any other developing organ
Oncology:
-Tumor detection and tumour burden quantification
-Visualization and quantification of tumour angiogenesis, vascularization and
perfusion
-Therapy responses (non invasive measure of tumors over time)
-Guided implantation of tumor cells
-Precise injection of drugs into tumors
-Ultrasound guided biopsy
Abdominal analysis:
-Function of kidneys, liver and reproductive system
Cardiovascular studies:
-Cardiac function in 2D, 3D or 4D
-Hemodynamics
IVIS Lumina XR
Real-Time in vivo bioluminescence, fluorescence & X-Ray Imaging System for
small animals.
• General characteristics:
– 10 filter sets to image reporters that emit from green to near-infrared
(capable of imaging all common fluorescent and bioluminescent reporters).
– Heated stage to keep animals from becoming hypothermic.
– Input and output connection ports for the anesthesia system.
– X-ray. Precise optical and X-ray overlay allows you to bring your optical
signal into.
• Applications:
The IVIS Lumina XR can be used in oncology research to follow tumor
progression, monitor response to therapeutic treatments, metastasis, cell
tracking, and tumor burden monitoring.
Other uses include: infectious disease, inflammation studies, metabolic
disease, neurology, gene therapy, stem cell biology, cardiovascular disease,
immunology, transplantation biology, and drug metabolism studies.