HomeAbout the InstituteResearch UnitsPeopleLinksPh.D ProgramEnglish Language Idioma EspañolWeb SearchIntranet Access
 
 
Research highlights
Repression of Irs2 by let‐7 miRNAs is essential for homeostasis of the telencephalic neuroepithelium.

Virginia Fernández, Maria Ángeles Martínez‐Martínez, Anna Prieto‐Colomina, Adrián Cárdenas, Rafael Soler, Martina Dori, Ugo Tomasello, Yuki Nomura, José P López‐Atalaya, Federico Calegari, Víctor Borrell.

EMBO J 2020; e105479
doi: 10.15252/embj.2020105479
Published: 29 Sept 2020

link video Video "Micro RNAs son esenciales para el desarrollo del cerebro" »

Press release (Spanish) »

Structural integrity and cellular homeostasis of the embryonic stem cell niche are critical for normal tissue development. In the telencephalic neuroepithelium, this is controlled in part by cell adhesion molecules and regulators of progenitor cell lineage, but the specific orchestration of these processes remains unknown. Here, we studied the role of microRNAs in the embryonic telencephalon as key regulators of gene expression. By using the early recombiner Rx‐Cre mouse, we identify novel and critical roles of miRNAs in early brain development, demonstrating they are essential to preserve the cellular homeostasis and structural integrity of the telencephalic neuroepithelium. We show that Rx‐Cre;DicerF/F mouse embryos have a severe disruption of the telencephalic apical junction belt, followed by invagination of the ventricular surface and formation of hyperproliferative rosettes. Transcriptome analyses and functional experiments in vivo show that these defects result from upregulation of Irs2 upon loss of let‐7 miRNAs in an apoptosis‐independent manner. Our results reveal an unprecedented relevance of miRNAs in early forebrain development, with potential mechanistic implications in pediatric brain cancer.



Research Highlights Archive

CSIC-UMH
 
 
Consejo Superior de Investigaciones Científicas
Universidad Miguel Hernández

Campus de San Juan | Sant Joan d’Alacant
Alicante | España

in@umh.es
Tel. + 34 965 23 37 00
Fax + 34 965 91 95 61
© 2004-2020 Instituto de Neurociencias
Alicante | España | Legal Note | Mapa Web
Diseño web Digital Nature